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The nonglycosylated form features a lower stability when compared to the glycosylated form
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Similarly, the free energies of unfolding of C^sub H^2 antibody domain and pliage P22 coal proteins are, respectively, 3
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Denaturation of proteins, whether to a totally unfolded conformation or to a partially unfolded molten globule, has been shown to be reflected in increased atomic thermal motions relative to the native state by several techniques, including NMR (Idiyatullin et al
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A trace from a fluorescence anisotropy decay measurement of aggregates (incubated under A-state conditions for 24 h) is shown in Fig
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In addition, slower multiphase unfolding is detected at higher denaturant concentrations
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BD=body depth; BL=body length;ED=eye diameter; HD=head depth; HL=head length; PAL=preanal length
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Challenges (all covered below) include precisely controlling lubricant mixing systems, maintaining thermal stability, choosing the appropriate cutting tools and removing the chips
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Such analysis nonetheless enables us in the current study to estimate the entropic contribution of subsets of atoms, or degrees of freedom of interest, down to residue and atomic levels
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Functional and immunological characterization of human-immunodeficiency-virus type-1 envelope glycoproteins containing deletions of the major variable regions
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, 1998), as well as the four-way helical junction from which it is derived (F
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More recently, single-molecule FRET detection (Ha et al
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A peak close to zero FRET efficiency is always observed, and occurs in part due to photobleaching of the acceptor, as well as other processes that give rise to donor-only molecules (Deniz et al
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