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. Linear regimes are hard to identify
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. Enzyme Structure and Mechanism
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. Here, we have used fluorescence methods to follow the membrane association of a chimeric PLCβ/δ, referred to here simply as PLCβ (see Methods) under pressure
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. The first peak is centered at 28°C and reflects DMPC phase transition in intact disks that is not detected by CD (Fig
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. Disk fusion is accompanied by dissociation of lipid-poor monomolecular apoA-2, as indicated by the low-molecular-weight band corresponding to the particle size of ~7
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. The Θ^sub 222^(t) data show a rapid signal loss at any Gdm HCl concentration, indicating fast unfolding within the dead-time of the experiment (30 s), which precludes its quantitative analysis; the amplitude of this rapid phase increases with increasing denaturant concentration (Fig
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. In our model, cratic entropy reflects the fact that multiple protein molecules are held together on the disk (low-entropy state) but dissociate in solution (high-entropy state)
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. Therefore the conserved A6-A11 SS-bond is expected to cause a significant topological constraint and bending of the A-chain
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. 99 w/d38Table 5Positive stations for posttransition pinguipedids in the Argentine Sea,during 1992-2001
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. An epitope recognized by the monoclonal antibody MR-21 which is reactive with the cell surface of Chattonella marina type II
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