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. , 1999), easier translocation of the molten globule through membranes compared to fully folded globular proteins (Bychkova et al
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. Parameter A, which reflects the spectral shape of the intrinsic Trp fluorescence (31), was obtained by dividing the fluorescence intensity at 320 nm by the intensity at 365 nm
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. and the Ni^sup 2+^-resin was from Imitrogen (Carlsbad, CA)
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. Usually, it is possible to distinguish between these limits by the number of resonances per exchanged spin, present in the NMR spectra
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. Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules
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. Many of these have been considered in greater detail in previous columns
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. Among first fibrillar features, short but relatively thick (8-nm) seedlike forms appeared on a very short timescale within the first minute of incubation
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. Unfortunately, not all individuals caught during the cruises were examined by us; therefore, to avoid biases caused by identification errors, the posttransition juveniles of both species were considered as a group
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. This issue and appropriate control experiments are discussed further in the Results section
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. Epidermal growth factor receptor is also expressed on the cell membrane, which shows homology to the transforming protein of the avian erythroblastosis virus (v-Erb-B) at the amino acid level
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. (1-8) Most victims (64%) are taken to an emergency department in a private vehicle; as many as 8% are transported by emergency medical services
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. A 100 µl sample of Hoechst (1 µg/ml) was added to each of the wells
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. The interaction was again not significant, F (1,140) = 2
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. In high concentrations of denaturants, proteins behave like polymers in a good solvent and ensembles for denatured proteins can be modeled by ignoring all interactions except excluded volume (EV) effects
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. The dimerization surface included several salt-bridge networks, such as Asp^sup 467^-Arg^sup 424^', Arg^sup 456^-Asp^sup 358^', Asp^sup 540^-Arg^sup 422^', Asn^sup 345^-Asp^sup 448^', as well as backbone-backbone hydrogen bonds (Fig
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. The MR characteristics in the animal and vegetal cytoplasm also differed from each other: At identical voxel size, the integral of the triglyceride peaks in the vegetal cytoplasm was two to three times the value found in the animal cytoplasm, with the actual ratio varying between individual oocytes
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. This view is supported by both the signal amplitude and linewidth in the nucleus with time: Depending on the binding strength, bound molecules would give broad NMR peaks or no signal at all, and the diminazene signal is initially (after 1 h) much smaller than that of aceturate but, after 6 h, reaches ~½ of the aceturate amplitude
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.5 Our previous studies6,7 have focused on the consumption of base metals such as Cu and Ni-P, and it is found that SnAg solder consumes a higher base metal than the SnCu solder system, whereas a moderate amount of base metal consumption is pronounced for the SnAgCu solder system
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. The liquid-liquid coexistence region and glass transition are well separated, and so the two processes will not affect one another
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. The spectrum of lipid-free apoA-2 at 25°C is invariant in the protein concentration range from 5 to 20 µg/ml and suggests 30% α-helix (Fig
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. Helical junctions as determinants for RNA folding: origin of tertiary structure stability of the hairpin ribozyme
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