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. As shown in Fig
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. Given the equivalence between the EV limit and highly denatured states, our ability to simulate conformational ensembles in the EV limit, with full atomic detail, has direct bearing on the development of an accurate physical picture of conformations accessible to denatured proteins
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. The results of this study suggest that the ANS binding method may find practical utility in testing the effectiveness of various additives employed in protein formulations as well as to devise safer freeze-drying protocols of pharmaceutical proteins
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. No model based on protein conformation distribution was used to analyze or interpret these results in more detail; however, such decay times and relative amplitude to the overall fluorescence decay are useful parameters when comparing samples of different chemical history or taken as well as in interpreting relative contributions to steady-state fluorescence data
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. Interestingly, a lack of enhanced membrane binding was also observed for the nonspecific PLCβ-membrane association, suggesting that the enzyme packs efficiently with the membrane interface
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. A new aspect is the formation of so-called superfibrillar aggregates as shown in Fig
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. The enzymes were dissolved in 30 mM Tris-HCl, pH 7
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. If one were to zoom into the contact density map of a long protein such as titin one reproduces the contact density map for a shorter protein such as uhiquitin or peripheral subunit binding domain (PSBD)
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.Key words: asthma; COPD; cystic fibrosisAbbreviations: CF = cystic fibrosis; CFTR = cystic fibrosis transmembrane regulator; Gas6 = growth-arrest-specific-factor 6; GTPase = guanosine triphosphatase; HGF = hepatocyte growth factor; IL = interleukin; LRP = lipoprotein receptor-related protein; MFG = milk-fat-globule-factor; PPAR = peroxidase proliferator-activated receptor; PS = phosphatidylserine; PSR = phosphatidylserine receptor; SP = surfactant protein; TGF = transforming growth factor; TNF = tumor necrosis factor; VEGF = vascular endothelial growth factor**********Cell death, removal, and replenishment are increasingly recognized as fundamental processes in both the developing and the developed organism
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. Mechanisms of tryptophan fluorescence shifts in proteins
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. The activation energy E^sub a^ was determined from the slope of these plots with the accuracy of 5-8 kcal/mol, which includes the fitting errors and the deviations among different data sets
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. Although the exact time of disk reconstitution varies for apA-2 from different pools, our data consistently show that the vesicle-to-disk conversion after heat denaturation of apoA-2:DMPC disks is a slow reaction that takes hours to complete at 24°C
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. 1, q and r) clearly show domains of parallel fibrils with little tendency to criss-cross
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. The E values of these two conformations were set to 0
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. Under its most restrictive form--Type 1 RP--only chemically equivalent drugs (i
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.023 -7,634 -590Cumulative effect post-RP-29,812
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