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. Nevertheless, the 2D IR analysis also suggested that the pretransitional conformational changes involved the unfolding of the solvent-exposed β-sheet structures and aggregates appearing at a temperature at which the protein had not fully unfolded
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. However, the absence of native baseline at low temperatures did not yield reliable results when fitting the data to Eq
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. J Immunol 2006; (in press)(57) Lee JH, Lee DS, Kim EK, et al
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. Similar microscopic measurements and analysis were performed in solutions of PEG with a higher molecular weight and the same PEG concentration as used in the above experiments
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. [infinity] = asymptotic length; k -- Brody growth parameter; [t
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. The simulations incorporate peak broadening due to interconversion of the proposed extended undocked and quasi-docked conformations (Fig
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. Why is one Bacillus alpha-amylase more resistant against irreversible thermoinactivation than another? J
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. The different voxels, however, delivered indistinguishable spectra
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. Three probes-ANS, DCVJ, and ThT-show increased fluorescence throughout the aggregation reaction, whereas Bis-ANS shows fluorescence decay (Fig
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. We remark that in both these cases, the stabilized hypergutter provides an arena in which "diffusion-collision" calculations can operate within a molten globule, so constituting a significant generalization of that model to nonspatial degrees of freedom (Zhou and Karplus, 1999)
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.The model provides an alternative interpretation of the results of protein engineering analysis, and implies that not all contributions to measured Φ-values at the transition state may arise from native-like interactions
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