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. Compact state of a protein molecule with pronounced small-scale mobility: bovine alpha-lactalbumin
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. When excited at 280 nm, gSBA shows a fluorescence emission maximum at 328 nm, which is red-shifted to 354 nm on denaturation with 7
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. Out of the 27 hydrogen bonds, only two interactions are found to be intrasubunit (i
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. The intermediate and high q regimes provide the most information regarding average chain shape and fluctuations (62-64)
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. Although glycosylation does not affect the structure, protein dynamic fluctuations decrease globally, decreasing amide hydrogen exchange rates by up to sixfold throughout the entire protein
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. As in the case of the Φ-value analysis, this experiment is also usually interpreted as giving information about the transition state (Oliveberg et al
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., 1996) and the extension or stretching of the coiled-coil gp41, leading to the insertion of the membrane fusion peptide into the host membrane, which induces fusion of the viral and host membranes (Chan and Kim, 1998)
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