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Then, we centralized the maintenance of that information at the corporate office
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. Trypsin digestion experiments EI^sup sc^ was mixed with trypsin (al linal concentrations of 1 µg/µl for El^sup sc^ and 0
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. Similar results have been observed in 1), the enzyme malate synthase G (54), a close structural analog of pyruvate dikinase and EIC (11), where binding to pyruvate does not cause large structural changes (55); and 2), the pyruvate dikinase from maize, where only small changes are observed in selected regions upon binding of PEP (56)
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. These changes in the thermodynamical parameters point to subtle conformational variations in the packing of some residues
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. Sample preparation Before sample preparation, all protein stocks were dialyzed against 2 mM Hepes buffer, pH 7
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. For lipid T^sub 2^ measurements in the cytoplasm, localized spectroscopy with (200 µm)^sup 3^ voxels was employed with 10 different echo times in intervals of 4 ms, starting at 6
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. Moreover, the surface tension of tin, lead, and solders generally decreased with the increasing temperature and showed a continuous smooth exponential function from the high surface tension component (tin) to the low (lead)
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. The rate determining step is not the rapid formation of local secondary structure, nor the diffusion of subdomains per se, but the organization of large pieces of secondary structure into the same topological configuration as the native state, which thereafter is able to form rapidly
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. CD measurements suggested that unfolding of GR was complete at 7 M GdnHCl, a result that was consistent with the iodide quenching data
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. 4 strongly supported the conclusion that pretransitional conformation changes occurred at temperatures where the enzyme retained high activity (see Fig
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. However, our assessment of contact density maps, difference contact maps, and topological measures clearly indicate that highly denatured states, which are characterized by dilatation symmetry, are topologically distinct vis-à-vis their native-state counterparts
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. 5 b, phase AFM images of the morphological features obtained after 5 min of the 60°C incubation are shown
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