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. Protein structure and dynamics at high pressure
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. These observations in the IR spectra coincided with the deduction based on DSC results that the thermal unfolding of ACYI is an irreversible process accompanied with protein aggregation
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. The main contribution of field theories is the recognition of the special properties of conformational ensembles that are encoded by correlated fluctuations imposed by the self-repelling nature of a polymer in the EV limit
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. After eliminating the denaturing force the proteins are not able to fold back into their native (and functional) structure and the still unfolded proteins form (or just stay on with) aggregates
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. As a first step in this direction, we measured the thermal unfolding transition of α-amylases in the presence of NDSB, which is known to be an effective agent to prevent aggregation of thermal unfolded proteins (32-34)
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. © 2005 by the Biophysical Society0006-3495/05/08/1227/10 $2
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. 6 a shows a representative example of the morphological variability in insulin fibrillar aggregates grown for 10 min at 70°C
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. The conformational sampling requires more time than for CD4
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