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. The transition curves of the amide I' hands were obtained according to a method described previously (28)
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. A summary of our results from analysis of EV-limit ensembles for 23 different two-state proteins is provided below: 1
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. Key words: asthma; COPD; cystic fibrosisAbbreviations: CF = cystic fibrosis; CFTR = cystic fibrosis transmembrane regulator; Gas6 = growth-arrest-specific-factor 6; GTPase = guanosine triphosphatase; HGF = hepatocyte growth factor; IL = interleukin; LRP = lipoprotein receptor-related protein; MFG = milk-fat-globule-factor; PPAR = peroxidase proliferator-activated receptor; PS = phosphatidylserine; PSR = phosphatidylserine receptor; SP = surfactant protein; TGF = transforming growth factor; TNF = tumor necrosis factor; VEGF = vascular endothelial growth factor**********Cell death, removal, and replenishment are increasingly recognized as fundamental processes in both the developing and the developed organism
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. Proteins in high concentrations of denaturants, such as 8 M urea or 6 M GdnCl, behave like chains in good solvents (3)
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. A twofold advantage underlies our choice of sort-core repulsions
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. This scale invariance, referred to as dilatation symmetry (39) is a hallmark of chains in the EV limit and rollouts the preservation of the hierarchical nature of contact patterns irrespective of sequence or chain length
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. A new perspective on unfolded proteins
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. Taking advantage of the subcellular resolution now achieved with MRS, we examined, upon drug delivery, the spectroscopic changes in the three subcellular compartments of the Xenopus oocyte over time
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.Chemical cross-linkingA total of 100 µl of aliquots from a TTR misfolding reaction was withdrawn al different time points, and 100 mM phosphate buffer was added in addition to 10 µl of glutaraldehyde (25%, electron microscopy grade (Sigma, St
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. Membrane association of Gβγ can be monitored by changes in emission of a covalently linked CPM probe (Fig
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.The method was also successful with 102 eggs collected from Wakasa Bay (Table 4), which had been immediately fixed with 5% formaldehyde in seawater
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. The loss of these specific tertiary docking interactions makes these SL constructs mimics for the undocked state of the corresponding WT ribozymes
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. Conversely, pellets maintained in DMEM/10%FCS possessed limited extracellular matrix (Fig
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. The codes for these supplying fees are G0370 and G0369, respectively
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