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The fully deuterated samples were obtained by incubating the protein solutions in a water bath at 50°C for 15 min, and then the samples were cooled to room temperature and lyophilized group health insurance dallas .
Partial motivation for this reference line comes from the work of Zagrovic and Pande (78), who showed that internal distances in unfolded ensembles of several proteins follow the predictions of the ideal random-flight chain with link length of 3 group health insurance dallas .
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The three wavelengths yielded the same unfolding curves (data not shown) group health insurance dallas .
Crystal structures of pyruvate phosphate dikinase from maize revealed an alternative conformation in the swivelling-domain motion group health insurance dallas .
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This is both natural and necessary, since data from kinetic experiments do just the same, but there is a danger in overlooking aspects of folding that rely essentially on the presence of many degrees of freedom group health insurance dallas .
By fitting the peaks in the histograms with Gaussian functions, we characterize their properties, such as peak position, relative areas of the peaks, and peak shapes as described in the Materials and Methods section group health insurance dallas .
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