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That the intermediate is monomeric in nature is also evident from its fluorescence emission λ^sub max^, which is similar to its fully folded monomeric state, characterized earlier (23) (Fig health alternative herbs .
and those of Wilkins et al health alternative herbs .
42 kcal mol^sup -1^) and hydrophobic contacts (ΔΔG = + 4 health alternative herbs .
This value (311 ± 5 K, at 4 µM protein concentration, 315 ± 9 K, at 6 µM protein concentration) was similar, within the experimental uncertainty, to that obtained in the intact EI^sup ec^ (4), where two tryptophan residues are located at the C-terminal region of the protein health alternative herbs .
As already mentioned above, there is indication from previous studies on several α-amylases that a structural unfolding is followed by an irreversible process (14,22-25) health alternative herbs .
IENS is, therefore, well suited for experimental investigations of thermal fluctuations within biological macromolecules under different biochemical conditions on timescales that match those of MD theoretical calculations health alternative herbs .
Fluorescence spectroscopy provides a very attractive alternative for such studies, due to sensitivity, ease of use, and flexibility health alternative herbs .
0) or 25 mM HCl (pH 1 health alternative herbs .
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The fixed eggs were transferred to net wells (mesh size 200 [micro]m, diameter 24 mm, Corning Incorporated, Corning, NY) and washed with 10 mL of distilled water three times health alternative herbs .
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The specimens were placed into embedding moulds and cured for 18 hours at 70°C health alternative herbs .
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