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. Details of these tests for convergence of the simulations and the sample size are presented in the Appendix
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. To calculate the form factor we used the positions of α-carbon atoms for each residue
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. Comparison of results in Fig
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. the tertiary structure of EI^sup sc^ remained unaltered, as suggested by fluorescence and ANS-binding (Fig
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. Inspection of the EIC^sup sc^ (Fig
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. Cell 2005; 123:321-334(12) Walsh GM, Sexton DW, Blaylock MG, et al
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. Mutation studies on E12 (a bHLH protein similar to SEF2-1) have shown a gradual decrease in CaM affinity upon each mutation of an interacting residue, and not the on-off behavior usually seen with lock-and-key or induced-fit binding mechanisms (15)
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. Purification was performed as described previously (Lashuel et al
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. In addition, apoA-2:DMPC disks were prepared by cholate dialysis (Jonas, 1986); EM, gel electrophoresis, and spectroscopic data of these disks and of the disks formed by spontaneous reconstitution were similar
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. Preorganized secondary structure as an important determinant of fast protein folding
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. In this procedure, the 50 µs data was first rebinned in 500-µs time bins, and rebinned data points lying above a threshold of 40 counts were identified (i
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. consumer spending at casinos in 1994$28
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. IR (Infrared) suppressors were designed into the engines to channel ambient air into the exhaust that helps reduce the IR signature
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- Consider a few examples of churches striking real-estate gold:* In Chicago, a 60-story condo tower will rise above St
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. N-Acyl-L-aromatic amino acid deacylase in animal tissues
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.uk) by using the Needleman-Wunsch global alignment algorithm (with the following parameters: matrix: EBLOSUM62, gap penally value: 10
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. In essence, the structure consisted of an α-helix subdomain, which is a four-helix bundle, comprising α-helices 1-4; an α/β subdomain, comprising a β-sandwich, formed by a four-stranded parallel β-sheet (β-strands 1-4) and a three-stranded antiparallel β-sheet (β-strands 1, 5, and 6), and three short helices (α-helices 5-7) that contain the phosphorylation site (His^sup 186^) (Fig
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.Efferocytosis is impaired in several COPD animal models, including SP-D knockout mice (24) TNF-[alpha]-overexpressing mice (K
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. Nature 2002; 418:200-203(20) Stuart LM, Ezekowitz RA
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. Ua and a^sup /^), whereas increasing the solder bath temperature to, say, 295°C results in a decrease in the number but an increase in the size of the IMCs (Fig
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. Fluorescence spectroscopy provides a very attractive alternative for such studies, due to sensitivity, ease of use, and flexibility
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. We have previously found that activated PLCβ-Gβγ complex can be rapidly deactivated by Gα(GDP) subunits without dissociation, which led to the suggestion that Gα(GDP) binds to PLCβ-Gβγ and perturbs the activating interaction without significantly affecting the PLCβ-Gβγ binding energy
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.The molecular details of how PLCβ interacts with Gβγ is unknown
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. The fluorescence emission was collected using the same objective, separated from the excitation light using a dichroic filter (540 DRLP, Omega Optical, Brattleboro, VT), spatially filtered using a 100-µ pinhole, and separated into donor and acceptor components using a second dichroic filter (630 DRLP, Omega Optical)
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. Ligand-induced conformational changes observed in single RNA molecules
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. "Tighter payments for service, like tighter payments for prescriptions, are part of a road and fundamental trend in health care systems around the world," he noted
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. These portraits inevitably fail to discuss their subjects in context, fail to examine the sheer pathology of their characters, and in the resultant glibness they end up supporting the dominant culture's tacit assertion that fast cash, no matter how dismally gained, is the American dream towards which all lives should be directed
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