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. Internal distances between residues that are beyond l^sub s^ should show the same power law dependence on sequence separation as does R^sub g^ (or R^sub e^) on chain length (N)
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. We note here that although we have interpreted peak shifts and broadening in terms of distance changes and ribozyme subpopulations, respectively, peak shifts could also potentially be caused by solvent effects on dye photophysics, and corresponding changes in the correction factor [gamma] (see Materials and Methods), as well as peak broadening by effects of [kappa]^sup 2^, the orientation factor (Deniz et al
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. Snapshots were saved for analysis once every 10^sup 3^ trial moves
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. For each of the amino acid sequences shown in Table 1, ensemble averages and conformalional distributions were obtained from an ensemble with a sample size of 10^sup 4^ and the ensembles were generated as discussed above
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. In other words, chains in the EV limit are not hard, prolate ellipsoids
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. A possible explanation for this large red shift during thermal unfolding, which is almost as large as those obtained with GndHCl-induced unfolding (see Table 2), may be given by the fact that the absent aggregation gives rise to only a weak burial of tryptophan residues
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. For example, Fourier transform infrared spectroscopy experiments when conducted on freeze labile proteins have not detected significant changes in their secondary structure (2) presumably because they are carried out at relatively large protein concentrations, a condition in which the perturbation is often attenuated as if the protein itself acted as a stabilizer
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. Such a correlation diagram of R^sub ex^ and ΔR^sub ex^ is shown in Fig
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.Since the docked and undocked peaks are well resolved, any interconversion between the two conformers must occur more slowly than the ~280-µs average burst duration of the single-molecule measurements (see below)
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