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The protein was expressed and purilied as described (23) health clinic .
Conversely, in the presence of PEP (either alone or with Mg^sup 2+^) health clinic .
the intensity of the fluorescence spectra decreased (Fig health clinic .
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The results of this study suggest that the ANS binding method may find practical utility in testing the effectiveness of various additives employed in protein formulations as well as to devise safer freeze-drying protocols of pharmaceutical proteins health clinic .
The native state of HuBChE is also stabilized by the kosmotropic sodium phosphate buffer; its flexibility is reduced and its resilience increased over a large temperature range as established by neutron scattering (44) health clinic .
It is noteworthy that all G-protein subunits are posttranslationally modified with hydrocarbon chains (see Wedegaertner et al health clinic .
The reaction rate is even slower above 24°C, suggesting that the heat-induced disk-to-vesicle conversion is thermodynamically irreversible health clinic .
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We also used ANCOVA to test for a maturity stage effect in the relationship between absolute fecundity and fork length health clinic .
0001,) of a stage effect (i health clinic .
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The mean age of the sample was 26 health clinic .
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