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. Assuming an average lifetime of 3 ns for Trp in aqueous solution (Ferreira, 1989), the resulting value of K^sub SV^ for iodide quenching of Trp is 19
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. The converse was observed for type-I collagen immunolocalisation, with positive staining evident within pellets maintained in DMEM/10%FCS (Fig
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.To characterize in more detail the structural changes induced by GdnHCl, we have investigated the accessibility of thiol groups of GR (which contains six Cys residues per subunit) to modification with DTNB (Fig
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. self-association of EI^sup sc^ could be mapped by some of its fluorescence residues (one tryptophan and eight tyrosine residues); and
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. at both excitation wavelengths, showed a bell-shaped profile, with a maximum at neutral pH (Fig
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. For this final state we do not watch a significant difference between formerly thermal or GndHCl-induced unfolded states
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