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Two intermediate states were populated at 2-3 M GdnHCl, as indicated by a combination of spectroscopic and chromatographic methods health food store edmonton .
14), also known as Acylase I (ACYI), is a homodimeric enzyme containing one zinc ion per subunit (4-6) health food store edmonton .
The thermal unfolding of ACYI might be a three-stale process involving an intermediate appearing at ~68°C health food store edmonton .
Consistent with previous observations (26), the intensity of the intrinsic Trp fluorescence decreased with the increase of temperature, and meanwhile, the emission maximum wavelength red shifted from ~333 nm to 340 nm (Fig health food store edmonton .
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