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. The folding of ACYI has been characterized to be a three-state process involving an inactive dimeric intermediate with molten globule-like characteristics (18,19)
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. The N [arrow right] I transition, which was referred to as the pretransitional change, ended at ~68°C and the T^sub 1/2^ occurred at ~60°C (see Table 1)
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. The spectrum of lipid-free apoA-2 at 25°C is invariant in the protein concentration range from 5 to 20 µg/ml and suggests 30% α-helix (Fig
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. 2) than those with apoA-1 (data not shown)
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