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. No changes in the elution volume of GR were observed when the sample contained 1 M GdnHCl
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. Among a large variety of different methods, such as absorption-, circular dichroism spectroscopy, calorimetry, NMR, and light scattering techniques, in particular fluorescence spectroscopy is a powerful technique to monitor unfolding transitions and to characterize properties of the unfolded state (see for example (3,4))
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. The ^sup 15^N R^sub 1^ and R^sub 2^ experiments at each field were recorded in an interleaved manner with the relaxation delays randomly distributed
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. Although the blank fluorescence for DCVJ is low, DCVJ in the presence of unfolded TTR shows even lower fluorescence than the blank, which could indicate a small quenching effect through binding
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. In the same way, if denaturant concentration affects the stability of the low-dimensional search spaces, or gutters, that are principally responsible for controlling the folding rate, then these experiments will also carry signatures of the search-structure
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. SUPPLEMENTARY MATERIALThe following supplementary material is available for this article online: TABLE S1
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. With the exception of LiCl, the sequences of salt activities are generally similar, and increase from Na+ toward ions with higher molecular weights
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. When studying the response of DNA to chemicals in solution, we always deal with elongated DNA, and under these conditions monovalent cations in free or DNA-bound states are substantially hydrated
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.([c] 2005 Book News, Inc
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