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He adds that many analysts and investors are unaware that companies often include nonrecurring items in their OCF
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. 5 M, the GR dimer eluted at a volume corresponding to ~120 kDa
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. Structural rearrangements then take place at the dimer interface (and presumably also in other domains of each subunit), leading to a final, compact dimer structure
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. 4 strongly supported the conclusion that pretransitional conformation changes occurred at temperatures where the enzyme retained high activity (see Fig
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. Toward an Accurate Theoretical Framework for Describing Ensembles for Proteins under Strongly Denaturing ConditionsTran, Hoang TABSTRACT Our focus is on an appropriate theoretical framework for describing highly denatured proteins
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. In high concentrations of denaturants, proteins behave like polymers in a good solvent and ensembles for denatured proteins can be modeled by ignoring all interactions except excluded volume (EV) effects
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. Second, the cavity statistics are indicative of large-scale correlated fluctuations, which exist on all length scales in the EV limit
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. The partial solvent exposure of this hydrophobia surface (due to protonation of some aspartic residue) could then cause binding to ANS
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. Extracellular-intracellular distribution of glucose and lactate in the rat brain assessed noninvasively by diffusion weighted 1H nuclear magnetic resonance spectroscopy in vivo
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. This is particularly intriguing, since its three-dimensional structure reveals that the active site of AChE, which, like other serine hydrolases, features a Ser-His-Glu catalytic triad, is buried at the bottom of a deep and narrow gorge lined by aromatic residues (26)
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. One of these, the interaction of CaM with basicHelix-Loop-Helix (bHLH) transcription factors has a 2:2 stoichiometry (11)
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. Comparison of the effective radius of sterically stabilized latex particles determined by small-angle x-ray scattering and by zero shear viscosity
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. ThT dramatically increases its fluorescence quantum yield when bound to amyloid fibrils; however, the mechanism behind this property is unknown
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. Fluorescence spectra were obtained from samples in quartz cuvettes with 10 mm or 4 mm pathlengths
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. Partially folded intermediates in insulin fibrillation
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. The cooling path for our non-equilibrium TIPS process is indicated by (1) [arrow right] (2), and the cooling rate is about 12 to 26°C/min near the cloud points
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. The proposed torsions are used to compute new Cartesian coordinates for the molecule
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. Indeed, engineered HuBChE with a reduced degree of glycosylation displays catalytic properties similar to those of the wild-type enzyme (62)
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. This is called "wraparound" binding because the two domains in CaM wrap around the α-helical target (8,9)
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.Deriving the activation barriers assuming a two-state exchange is likely to be a significant simplification, as the conformational landscape may be much more complex
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. This enzyme lacks the long C-terminal domain of PLCβ, which is necessary for activation by Gα^sub q^(GTP)
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. This duplex has a 14-bp separation between donor and acceptor, and spFRET experiments show only a small change in E upon going from 0
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