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that at 365 nm at different temperatures
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Interestingly, considerable conformational changes were observed before the enzyme was fully inactivated by high temperature
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5 b, but was also observed for all other α-amylases which otherwise exhibit aggregation upon thermal unfolding
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Therefore, a purely diffusion-controlled on-rate k^sub on^ of 10 s^sup -1^ M^sup -1^ would put a lower limit on the CaM:SEF2-1mp affinity, k^sub off^/k^sub on^ > 10^sup -6^
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3, A and B, suggests that the apoA-2 unfolding precedes lipoprotein fusion
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Polymer and solvent were mixed and heated to 160°C
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These data were used to estimate the location of the binodal curve so that we could better understand the effect of dissolving temperature on particle size
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Maturity stageMacroscopic descriptionHistological description1 ImmatureSmall and translucentOocyte cytoplasm intenselyovary, pink during monthsbasophilic
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We have successfully produced monoclonal antibodies to differentiate harmful marine phytoplankton species from morphologically similar harmless species (Hiroishi et al
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1973, Errington 1930, Lowe 1980, Raczynski and Ruprecht 1974), most have not (e
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2b), containing clearly defined collagen fibrils (Figs 5b and 5c)
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. The enzyme concentration was determined by measuring the absorbance at 280 nm and using the absorption coefficient A^sup %^^sub 1cm^ = 13
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. Details with regard to the intrinsic fluorescence experiments were the same as those described before (30)
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