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An additional experiment in which the incubation time of the protein in the presence of 3 M GdnHCl was increased from 90 min to 24 h showed that the small dimer fraction observed in Fig health individual insurance jersey new .
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Zaccai*||* Laboratoire de Biophysique Moléculaire, Institut de Biologie Structurale, Grenoble, France; [dagger] Centre de Recherches du Service de Santé des Armées, Unité d'Enzymologie, La Tronche, France; [double dagger] Institut de Pharmacologie et Biologie Structurale, Toulouse, France; § Division of Biochemistry, Walter Reed Army Institute of Research, Silver Spring, Maryland; ¶ Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel; and || Institut Laue-Langevin, Grenoble, FranceSubmitted February 9, 2005, and accepted for publication June 30, 2005 health individual insurance jersey new .
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Data analysisConversion of the apparent K(d) obtained on membrane surfaces to the K(d) that would be obtained if the proteins were freely diffusing has been previously treated by assuming that the proteins are confined to interact within a reduced surface volume (ν) that is equal to the surface area of the membrane multiplied by the distance from the membrane surface into the aqueous phase where the proteins interact, which is taken to be 50 nm health individual insurance jersey new .
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