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. At 3 M GdnHCl, however, a significant drop in ellipticity was observed
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. The attempts to avoid artifacts in the 2D IR plots have been described in detail elsewhere (30)
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. Finally, we studied the conformational and stability properties of EI^sup sc^ in the presence of the effectors (i
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. Intracellular monosaccharide and amino acid concentrations and activities and the mechanism of insulin action
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. K BeranSteed, and T
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. , Li and Kay, 1977; Sassaroli et al
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. The existing morphological studies on insulin aggregation stress the hierarchical intertwisting of protofilaments (Jimenez et al
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. The exponentially large search times arise transparently from the factor (R/R^sub N^)^sup (d-2)^ in Eq
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. semifasciata on the base of the caudal fin upper lobe (Herrera and Cousseau, 1996) was already present (Fig
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. The magnetite crystals are believed to have formed from the thermal decomposition of the siderite crystals in the reaction 3FeCO^sub 3^ -[arrow right] Fe^sub 3^O^sub 4^ + 2CO^sub 2^ + CO
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. Shearing the gel liberates discrete, spherical particles
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. [beta]0+[beta]1FL'where P(Y=1|FL) = probability of female black rockfish being mature at size FL; and[beta
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. Department of Commerce, under grant numbers NA36RG0451 (project no
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. Folding of the hairpin ribozyme in its natural conformation achieves close physical proximity of the loops
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. The mean age of the sample was 26
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. This suggests that the association of hydrophobic moieties might follow the disruption of secondary structures from N to A states
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. On the contrary, an important consequence of creating an internal cavity of 40 in I7S and 100 [Angstrom]^sup 3^ in F110S, presumably filled with water (18,19), is the onset of large amplitude structural fluctuations that increase remarkably the flexibility of the globular fold, as evidenced by the three to four orders of magnitude increase in permeability to acrylamide (18)
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. Although subcellular variations could not be determined with this whole-cell technique, it yielded spectra with increased resolution and sensitivity, since dipolar broadening and susceptibility variation within the sample are being reduced (30)
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. In the usual space-time windows of IENS experiments, this represents dynamics of larger atomic groups such as protein side chains (3)
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.Our main goal with this study was to employ a variety of fluorescence probes (ANS, Bis-ANS, DCVJ, and ThT) and techniques (steady state and time resolved) to recognize and characterize different states of a protein in the process of misfolding and self-assembly
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. Therefore, depending on internal packing, it could be approximately estimated that the thick fiber may consist of over a dozen of laterally associated protofilaments
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. One can, for example, resolve the entropic contributions of hydrophilic and hydrophobic residues to protein-protein binding, which is experimentally impossible
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.12 kJ K^sup -1^ mol^sup -1^; see Fig
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