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The fluorescence emission of native GR is quite blue-shifted ([lambda]^sub max^ at 317 nm), indicating that, on average, the four tryptophan residues in each GR subunit are well-protected from exposure to the aqueous medium in the folded protein health individual insurance virginia .
In brief, the intrinsic Trp fluorescence emission spectra were measured using a Hitachi F-2500 spectrofluoromeler (Tokyo, Japan) using 1-cm path-length cuvettes with an excitation wavelength of 295 nm health individual insurance virginia .
Since no high-resolution structure of ACYI is available, all of the Trp fluorophores (Classes A and S, I, II, and III) defined previously (34) were included in the fitting procedure health individual insurance virginia .
Attributes of Glycosylation in the Establishment of the Unfolding Pathway of Soybean AgglutininSinha, SharmisthaABSTRACT Soybean agglutinin (gSBA) is a tetrameric legume lectin, each of whose subunits are glycosylated health individual insurance virginia .
the changes observed should reflect conlormational rearrangements occurring in this region health individual insurance virginia .
We examined two single point cavity-forming mutants, I7S and F110S, whose internal cavities of 40 and 100 [Angstrom], respectively, appear to be fully hydrated (18), and the double mutant C3A/C26A in which the disulfide bridge linking strands β1 and β2 has been severed health individual insurance virginia .
Seung-Cheol Lee, Jee-Hyun Cho, and Daniel Mietchen contributed equally to this work health individual insurance virginia .
5), must be at a higher free energy than the native unfolded state, C (Fig health individual insurance virginia .
The wetting balance tests were conducted for Cu and Ni substrates with NC, R, and WS fluxes at 255°C, 275°C, and 295°C health individual insurance virginia .
The Trp fluorescence of acid unfolded TTR peaks at 352 nm (data not shown) compared to 340 nm in the native protein, indicating unfolding health individual insurance virginia .
Although the exact shape of this transition varies for apoA-2 samples from different plasma pools, the sharp peak at 24°C is always observed after disk heating beyond the high-temperature DSC transition, indicating disk-to-vesicle conversion in this transition health individual insurance virginia .
Red sea bream (Pagrus major) (Table 1) eggs can easily be distinguished from those of other sparids also found in Japan, such as Acanthopagrus latus, by differences in egg size and spawning seasons, and from those of Evynnis japonica by differences in spawning seasons (Ikeda and Mito, 1988; Kinoshita, 1988; Hayashi, 2000) health individual insurance virginia .
Identification of pelagic eggs of marine fishes by rearing method health individual insurance virginia .
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