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. These results suggested that during ACYI thermal denaturation, the dominant events were the unfolding of the β-sheet (1638 cm^sup -1^) and the formation of the intermolecular β-sheet in aggregates (1617 cm^sup -1^)
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. The recomhinant form also shows emission maxima at 327 nm for the native stale and 357 nm in the completely denatured state (Fig
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. There have been numerous attempts to develop models for conformations accessible to highly denatured proteins
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. This shape did not change as the pH was modified, except in the region between 3
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. 2 K; 4 µM, T^sub m^ = 329
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. This makes a direct determination of the rotational correlation time impossible
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. Influence of the nature of the donor particle interaction with the receptor
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. For if a residue contributes via nonnative interactions to the stable hypergutter that concludes the dominant (longest) diffusive search, then mutations to that residue will affect the folding rate, even though it does not necessarily possess any native contacts at the transition state
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. Particle formation depends on various solution parameters and cooling conditions
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. The peak positions for the SL 2WJ and 3WJ constructs closely follow the trends observed for the undocked peak with the corresponding WT constructs, confirming that removal of the loop on arm A provides appropriate models for the undocked states of the WT ribozymes
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. Since gSBA and rSBA both exist as tetramers with identical ligand binding specificities and macromolecular properties, the change of the mode of unfolding from a two-state process to a three-state process and the decrease of T^sub m^ by 26°C for the thermal unfolding of the protein of the kilter implicate the covalenlly linked glycans for the stability of gSBA
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. Experiments at pHAcrylamide quenching experiments, measured by excitation at either 280 or 295 nm, showed exponential Stern-Volmer plots (Table 1 ), which yielded similar results: the K^sub sv^ were larger at acidic than at neutral pH, and the experiments in GdmHCl had the largest values of K^sub sv^
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. The intraresidue conformational entropy obtained in this way provides, therefore, only information about side-chain motions
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