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. Notwithstanding the relevance of the phenomenon, little is known of the structure of proteins in ice owing primarily to the inapplicability or the poor sensitivity of usual spectroscopic techniques in this highly scattering and anisotropic medium
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. The plasmid carrying the WT sequence was a generous gift from Prof
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. (8) achieved magnetic resonance (MR) microscopic imaging of a large single cell, the Xenopus laevis oocyte whose diameter is ~1
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. Protein compactness measured by fluorescence resonance energy transfer
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. Facultad de Ciencias Exactas y Natutales, Universidad de Buenos Aires-INIDEP
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. For instance, the blue coloring of CG4's tail indicates the presence of tiny dust particles -- less than a millionth of a meter in diameter -- that scatter blue light preferentially, Malin says
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. In the diffusion spFRET measurements described in this article, by labeling the ends of the loop-bearing arms with donor and acceptor dyes, we use the FRET efficiency E as an indicator of the distance between arms A and B of the ribozyme during the docking process
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. Because the donor and acceptor are attached to the ends of the loop-bearing arms, a short donor-acceptor distance is expected if the two loops are in a docked conformation
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. Application of the Type 1 RP (generic substitution) produced annualized savings of $1 million, or roughly one-quarter of the savings realized by Type 2 RP
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. (Rudy had cynically labeled his own memo "Murder, Inc")
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. In spite of its harmful effects, the gene persists among human populations in parts of Africa with a high incidence of malaria
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.5 M GdnCl, which then increased at a higher concentration of the denaturant without the appearance of any intermediate
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. The probability of linding a pair of residues in close spatial proximity decreases with increasing sequence separation
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.Burying the dead : the impact of failed apoptotic cell removalon chronic inflammatory lung diseaseR
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. Some efferocytosis receptors appear to perform primarily a tethering function by holding the apoptotic cell and phagocyte close together (eg, CD14 or CD31), (19,21,22) while other receptors are primarily involved with transducing the engulfment signal (eg, LRP and PS receptors [PSRs]), (11,21,23,24) hence the so-called tether-and-tickle hypothesis (Fig 1, top, A)
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. Below the short Newtonian plateau, the viscosity increases with decreasing shear rate
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. SSA manifests itself through initial deposition of wild-type TTR amyloid fibrils in the heart
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. This analysis can potentially allow us to discriminate between A-state monomers, protein oligomers, and larger aggregates
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. Although the exact shape of this transition varies for apoA-2 samples from different plasma pools, the sharp peak at 24°C is always observed after disk heating beyond the high-temperature DSC transition, indicating disk-to-vesicle conversion in this transition
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. In this study, however, we have also observed, along the typical transient structural motifs, that lateral interactions normally dominating at the later stages of fibrillogenesis and responsible for the thickening of the fibers may, in fact, play a role at the very beginning of the process-and lead to an alternative subpathway of amyloid assembly
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. Water temperature at 10 m depth at positive stations varied between 12
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