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Pictured (l-r) are: Tom Graf, NRC President; Howard J
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Hence, these scans were not employed tor the calculation of the thermodynamic parameters for the unfolding of proteins
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A 1-cm-pathlength quart/, cell (Hellma, Muellheim/Baden, Germany) was used
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In general, the presence of Mg^sup 2+^ +, PEP, or both effectors together had a minor effect on the thermal stability of the protein as judged by the slight changes in T^sub m^ (the ΔΔT^sub m^s from DSC measurements equal + 1
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However, at least on a qualitative level in particular differences between several unfolded states can be detected easily
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Mutation studies on E12 (a bHLH protein similar to SEF2-1) have shown a gradual decrease in CaM affinity upon each mutation of an interacting residue, and not the on-off behavior usually seen with lock-and-key or induced-fit binding mechanisms (15)
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The system was dissected into various subsets of atoms to evaluate the entropic contributions of different functional elements
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The decision to provide full reimbursement for acetaminophen, ASA, ibuprofen, and naproxen was consistent with earlier recommendations by an independent academic research group, the BC Therapeutics Initiative, that these drugs be used as first line therapy for osteoarthritis (Therapeutics Initiative 1995)
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. The synchronous plot in Fig
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. The protein concentration used was 0
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. To probe the presence of aggregates, we employed chemical cross-linking using glutaraldehyde
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.RELATIONSHIP TO OTHER MODELSWe stress that the search-space picture explored above is not incompatible with the frameworks or results of the other models discussed in the introduction, but rather serves to show how the apparently alternative models are related, and how high-dimensional features are implicit in all of them
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. The apparent dissociation constants for Mg^sup 2+^ (K^sub D^^sup Mg^) recovered from the fits are 2
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. Hence, the 4WJ ribozyme is unique in its ability to fold into a structure with arms A and B in close proximity, similar to the native ribozyme, even in the absence of specific loop-loop docking interactions
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