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Thus, it seems reasonable to expect that the monomers of yeast GR may also be catalytically inactive and that dimerization may be critical for enzyme activity health insurance new student york .
5 C to avoid the possible artifacts caused by large band shifts accompanied with slight intensity changes in this temperature range (see also Fig health insurance new student york .
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The probable reason for the failure to deglycosylaic may be related to the fact that the terminal mannose residues are well rooted in the clefts and cavities on the protein surface, which made them inaccessible to the enzyme health insurance new student york .
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The k^sub ex^ derived from R^sub ex^ vs health insurance new student york .
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These fractions were plotted versus Mg concentration and fitted to the cooperative binding equation F = F^sup max^([Mg]^sup n^/([Mg]^sup n^ + (K^sup Mg^^sub D^)^sup n^)), to yield an apparent dissociation constant K^sup Mg^^sub D^for Mg^sup 2+^, and an apparent cooperativity coefficient, n health insurance new student york .
Clearly, the four-way helical junction in the native ribozyme is the most important determinant for proper folding, guiding the approach of arms A and B health insurance new student york .
Buckwalter JA, Mankin HJ health insurance new student york .
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