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. For each of the amino acid sequences shown in Table 1, ensemble averages and conformalional distributions were obtained from an ensemble with a sample size of 10^sup 4^ and the ensembles were generated as discussed above
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. Both the Flory random-coil model and field theories agree that all sequence-specific biases are strictly local and that denatured-state ensembles show significant conformational heterogeneity
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. INTRODUCTION The bacterial phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) is a multiprotein complex system that phosphorylates and, concomitantly, transports carbohydrates across the membrane into the cell (1)
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. DISCUSSIONThe effect of covalent inhibition by soman on the molecular dynamics of HuBChEThe structure and stability of the aged organophosphate conjugate of HuBChE produced by inhibition with soman are known to be modified compared to native HuBChE
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. The binding of soman to HuBChE via a single covalent bond (plus a salt bridge between a negatively charged oxygen on the phosphoryl moiety, and the protonated imidazole ring of His-438) may explain why it does not noticeably affect global HuBChE molecular dynamics
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. The inclusion bodies thus obtained were precipitated using 65% ice-cold acetone
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