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. Mutational analysis demonstrates that specific electrostatic interactions can play a key role in the denatured ensembles of proteins
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. 6 and a φ^sub max^ of ~0
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. AFM instrumentationAll AFM images were recorded on a MultiModc SPM microscope equipped with a Nanoscope IIIa Controller from Digital Instruments (Santa Barbara, CA)
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. Since overlap between blocks can reduce the standard deviation and hence the standard error (Fig
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. The results of ANCOVA over a common size range showed no evidence of differences in slopes between maturity stages (2-sided t-test P=0
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. The AFM-samples were dried gently with a nitrogen flow and left to dry overnight before the measurements, which helps improve the quality of the AFM data
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. The gp120 molecule without the V3 loop and the CD4 molecule, both using all atoms (code: all) or using only backbone (N, Cα, and C') atoms (code: bb); see Fig
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