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. DCVJ has not to our knowledge been used before on an amyloid system but has been employed on microtubule polymerization (Haidekker et al
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. , of bound and unbound states, whereas analysis of the single trajectories only provides information about the extent of the respective conformational spaces, and not about their degree of overlap
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. Native tetrameric TTR possesses two symmetric thyroxine binding cavities at the subunit interface (Fig
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