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"Once that's done, look at improvements that won't add a fixed future cost, such as employee bonuses and one-time improvements to technology or other essential machinery
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. The second term provides an estimate of the conformational entropy, which opposes chain swelling
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. Here, q is in units of inverse wavelength
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. FTIR The protein was speed-vacuum dried (Speed-vac rotatory evaporator, Savant Instruments, Farmingdale, NY) and dissolved in the deuterated buffer containing 10OmM NaCI
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. Molecular graphics were created with PyMOL (38)
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. Determining the magnitude of the fully asymmetric diffusion tensor from heteronuclear relaxation data in the absence of structural information
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. Changes in the 90° light scattering observed during heating and cooling of the apoA-2:DMPC complexes correlate with the CD changes (Fig
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. Throughout all experiments, only ultrapure water (resistivity > 18 MΩ cm) was used
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. This procedure also suggests a helpful way of conceptualizing links between the apparently divergent views of the folding funnel, collision-diffusion, nucleation-condensation and topomer search models
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. The MG concept was introduced to describe the properties of partially folded intermediate states sharing similar structural characteristics (i
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. The stability of GR dimers is also largely generated by the interface domain, since double or even single mutations in this region are sufficient to lead to the assembly of less stable dimers in the E
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. Of the numerous roles credited to glycosylation the major ones are to facilitate proper folding, prevent protein aggregation, protect against proteolytic attack, and act as surface cell receptors and targeting signals (4,5)
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. Ligand-protein contacts (LPC) was derived with LPC software: automated analysis of interatomic contacts in proteins
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. The CaM dimer is created by the tip of the N-terminal domain of one CaM monomer contacting the tip of the C-terminal domain of the other CaM
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. The resulting Θ^sub 222^(t) data, which showed CD changes during the dead-time of the experiment (Differential scanning calorimetryAn upgraded MC-2 microcalorimeter (Microcal, Amherst, MA) was used to record excess heat capacity of the apoA-2:DMPC complexes
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.Heating of apoA-2:DMPC disks from 5 to 70°C, i
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