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14), also known as Acylase I (ACYI), is a homodimeric enzyme containing one zinc ion per subunit (4-6)
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Stock solutions of guanidinium hydrochloride were prepared in 5 mM HEPES buffer, pH 7
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ANS binding studies were done on rSBA
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This is the case if at least one major component of the surrounding solvent is chemically equivalent to the main repeating unit of the polymer making chain-solvent contacts preferable to chain-chain contacts (4,37)
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For polymers in the EV limit, z [arrow right] ∞ and Flory's model is not applicable in this regime
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Specifically, we believe that ensembles for proteins obtained in the EV limit (48,124-127), will have characteristics that match predictions from field theories
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It may also be possible that a smaller degree of subunit dissociation in part explains the sharp increase in the residual enzymatic activity, retained after freeze-thawing, with increasing protein concentration (14,16,29)
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5 ms and two 180° pulses of 0
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The Sn-Ag system limits the solid solubility of Ag in Sn, which makes it more resistant to coarsening and forms more stable as well as uniform microstructure, whereas the addition of a small amount of Cu can prevent the dissolution of Cu from the base metal
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These data are consistent with previous reports which have demonstrated that neutral polymers such as PEG with a higher molecular mass are more effective for DNA condensation (Vasilevskaya et al
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Prefibrillar amyloidogenic oligomers and protofibrils of misfolded TTR were generated in vitro through induction of the molten globule type A-state from acid unfolded TTR through the addition of NaCl
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The spectra obtained upon Trp excitation (290 nm) are shown in Fig
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In the basal state, Gα(GDP) is strongly bound to Gβγ-subunits, but GTP-bound Gα has a much weaker affinity (e
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University of Illinois Press, Champaign, IL
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They differed from Parapercis spp
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Casing CaseinEighty percent of the protein in milk is casein
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Cheese, which contains little to no carbohydrates, is the original low-carb food
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R] Elite ABC kit is needed when a large number of field samples are analyzed
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HE TALKS UP THE COUNTY'S DRUG DIVERSION PROGRAM, PARTLY TO SHOW THAT LAW ENFORCEMENT ISN'T TOTALLY HEARTLESS
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"I know there are a lot of concerns about tighter reimbursement, rates for prescriptions," McClellan acknowledged
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In addition, the Pharmacy Quality Alliance will build a track record for the coming launch of Medication Management Therapy, which promotes appropriate medication use, reduces the risk of adverse events and optimizes therapeutic outcomes," he added
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. Do harshly denaturing environments such as 8 M urea or 6 M GdnCl mimic perfect solvents? In previous work, we developed a fast and accurate way to generate thermal, self-avoiding distributions for proteins with atomistic detail (48)
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. The distribution of end-to-end distances reveals the presence of a "correlation hole" as was first predicted by des Cloizeaux (41,111) and captures the diminution of entropy vis-à-vis the Flory random-coil estimates
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.Ca^sup 2+^ signaling can inhibit the transcriptional activities in vivo of the bHLH proteins E12 and SEF2-1 through direct binding of Ca^sup 2+^/CaM to the basic sequence of the proteins, resulting in inhibition of their DNA binding (15)
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. The mouse major urinary protein:pheromone interaction (48) and the topoisomerase I domain interaction with single-stranded DNA (49) have, or suggest to have, the aspect of multiple-binding alternatives in the bound state
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