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It's well known that in his crusade to pass the bill, DeLay drew on more than 800 pharmaceutical-industry lobbyists, millions of dollars in campaign contributions, and the efforts of numerous business and healthcare groups
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. Moreover, the existence of MG folded states in the unfolding of GR was indicated by HPLC studies (Fig
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. The transition curves of the amide I' hands were obtained according to a method described previously (28)
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. Earlier studies have shown that this protein shows exceptionally high stability in terms of free energy of unfolding when compared to other proteins from the same family
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. Sigmoidal transitions were only observed at pll 7 (23) and 9
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. Allograft tolerance induced by donor apoptotic lymphocytes requires phagocytosis in the recipient
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. Douglas), University of Colorado at Denver Health Sciences Center; and the Department of Immunology (Dr
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. In vitro mutagenesis and expression in Xenopus oocytes
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. ApoA-1, apoA-2, and other exchangeable apolipoproteins have evolved from a common ancestor that is related to the smallest human apolipoprotein, apoC-1 (57 aa) (Luo et al
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. To assess the high- and low-molecular-weight products of the thermal disruption and reconstitution of the apoA-2: DMPC disks, we used negative staining electron microscopy and non-denaturing gel electrophoresis (Fig
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. These forms vary from slight bends over double turns to semicircles of variable radii of curvature (not smaller than 250 nm)
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. It suggests that kinetic strategies for fast folding may be encoded rather generally in nonnative as well as in native interactions
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. For a molecule existing in two different states but with similar atom-positional fluctuations, similar configurational entropies are expected for the two states
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. Parametrization of aliphatic Clin united atoms of GROMOS96 force field
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. We calculated cooling rates as the slope of the average temperature between the top and bottom of a solution versus time at the cloud point of the 10 wt% solution (Figure 2)
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. Sexual maturityParameter values for the length-maturity logistic model were [beta
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. 0001,) of a stage effect (i
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. "We're talking about millions of lives saved
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. the refolding efficiency after unfolding rSBA with GdnCl was much less, as monitored from fluorescence emission scans
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.--Pharmacy Practice and Administrative Sciences Pharmaceutical Sciences* ILLINOISUniversity of Illinois at Chicago College of Pharmacy M
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.INTRODUCTIONProtein dynamics and function occur on timescales spanning several decades, from femtosecond electronic rearrangements (e
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.The crystal structures of Ca^sup 2+^/CaM show an extended dumbbell shaped molecule, in which its two globular domains are connected with a long α-helix (1)
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.A challenge faced by all these models is that the most successful search for inherent features of tertiary structure that correlate with folding rates has found that the topological measure of "contact order" is far more closely related than, for example, molecular weight itself in the case of "two-state" folders (Plaxco et al
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. In this method, the fluorescence of donor and acceptor are simultaneously recorded using avalanche-photodiodes as individual molecules traverse the excitation volume in a confocal microscope, and the FRET efficiencies from many such single-molecule observations are plotted in the form of a histogram
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. For both Cu and Ni substrates, R flux showed the worst performance
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