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The final enzyme concentration for the activity assay was 2 µM (~0 health mexico spa .
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5, C and D) and shorter fluorescence lifetimes (data not shown) reveal Trp to ANS and Bis-ANS fluorescence resonance energy transfer both in the native and misfolded states, indicating that the Tips and the probes are in close proximity (Conrad and Brand, 1968) health mexico spa .
PLCβ-Gβγ association was determined by the change in emission of CPM labeled on either of the two proteins as its unlabeled partner is added health mexico spa .
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