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The spectra recorded at temperatures from 68°C to 72°C were not included in the 2D IR analysis in Fig
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Molecular modeling of the SBA glycoprotein In the Protein Data Bank, none of the representative structures of soybean agglutinin show the entire covalently linked glycans
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These observations provide the necessary impetus for developing an accurate statistical thermodynamics framework for understanding how polypeptides respond to increasing concentrations of denaturants to yield ensembles that converge upon the EV limit description
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A spectroscopic and calorimetric investigation on the thermal stability of the Cys3Ala/Cys26Ala azurin mutant
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^sup 15^N chemical shifts were indirectly referenced using the gyromagnetic ratio of ^sup 15^N/^sup 1^H (18)
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The ^sup 15^N-{IH} nuclear Overhauser enhancement (NOE) shows only weak field dependence and was therefore only earned out at the higher field
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The two images present singular particles randomly distributed over the whole surface
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The large number of these, of order N times greater than the number of native interactions, make them a likely candidate for information storage, as well as their natural propensity for kinetic control
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Long-range electrostatic interactions beyond the cutoff were treated with a generalized reaction field model (Tironi et al
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Only scores of 2 and 3 were regarded as positive
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Sections were picked up onto copper grids, stained with 2% uranyl acetate and lead citrate and viewed on a Philips CM12 Electron Microscope at 80 kV
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--Pharmaceutical Sciences* FLORIDAFlorida Agricultural and Mechanical University College of Pharmacy and Pharmaceutical Sciences M
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3% of the females [C
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. These results suggested that during ACYI thermal denaturation, the dominant events were the unfolding of the β-sheet (1638 cm^sup -1^) and the formation of the intermolecular β-sheet in aggregates (1617 cm^sup -1^)
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. For the 28 proteins studied by Kohn et al
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. Although we predict similar volume changes for this association in the two different environments, the apparent affinities will differ because the effective concentration of membrane-bound proteins tends to be much higher due to their confinement on the reduced area of the membrane surface giving stronger apparent affinities (see Runnels and Scarlata, 1999)
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., the thickness of the DMPC bilayer) is 5
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. This protofilament + monomers/ oligomers scenario is an alternative pathway to the otherwise dominating protofilament + protofilament way of the protein fibrils' lateral growth
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