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A similar conclusion could he deduced from the intensity changes of the amide I' components shown in Fig
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In the interest of clarity, we show data for the sequence of ubiquitin
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Characlerization of the Exchcrichia coli enzyme I monomer/dimer transition kinetics by fluorescence anisotropy
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Among a large variety of different methods, such as absorption-, circular dichroism spectroscopy, calorimetry, NMR, and light scattering techniques, in particular fluorescence spectroscopy is a powerful technique to monitor unfolding transitions and to characterize properties of the unfolded state (see for example (3,4))
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5 T/m is required
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Estimating the time scale of chemical exchange of proteins from measurements of transverse relaxation rates in solution
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As with the PEG 3000 sample in Fig
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The aggregation process produced fairly monodisperse oligomers (300-500 kD) within 2 h that matured after 20 h into larger spherical clusters (30-50 nm in diameter) and protofibrils as shown by transmission electron microscopy
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Three probes-ANS, DCVJ, and ThT-show increased fluorescence throughout the aggregation reaction, whereas Bis-ANS shows fluorescence decay (Fig
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Lipoprotein fusion observed in our work is accelerated under denaturing conditions (Figs
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This suggests that on-line Basic advice had an impact on play, but that the Basic strategy was subsequently discarded after Block 4
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- I look at discharged-not-final-billed weekly from an inpatient and outpatient perspective
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