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. The N-terminal domain showed basically the same structure as the EIN^sup ec^ (8,9), since the alignments for homology modeling showed only minor deletions in loops between α-helix I and α-helix 2, α-helix 2 and α-helix 3, and α-helix 3 and α-helix 4 (Fig
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. Some efferocytosis receptors appear to perform primarily a tethering function by holding the apoptotic cell and phagocyte close together (eg, CD14 or CD31), (19,21,22) while other receptors are primarily involved with transducing the engulfment signal (eg, LRP and PS receptors [PSRs]), (11,21,23,24) hence the so-called tether-and-tickle hypothesis (Fig 1, top, A)
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. It is obvious from the data shown in Fig
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. Fluorescence measurements in ice were conducted as previously described (12)
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. Piazza and Iacopini's light scattering study using BLG A (39) showed a minimum in the second virial coefficient at pH 4
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. These results also suggest that current estimates of reproductive potential, in which fecundity for prefertilization females is used, may overestimate actual larval production because an increasing proportion of the stock consists of young fish
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. Ribozyme constructs missing the substrate strand were prepared in annealing buffer (missing NaCl and supplemented with 12 mM MgCl^sub 2^) at a concentration of 2 µM of each strand and annealed by heating to 70°C for 2 min and cooling to ambient temperature
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. Under reducing conditions, the number of SH groups in urea-denatured yeast GR increases to six SH groups/monomer (Massey and Williams, 1965), indicating full exposure of all the Cys residues in the protein
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. The mean residue ellipticity of the recombinant protein was similar to that of the wild-type, suggesting that there are no major structural changes between the native glycosylated and recombinant nonglycosylated proteins (Fig
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. Thus, in addition to the usual picosecond-timescale motions of varying contributions, a small amplitude nanosecond-timescale motion is present for all CaM residues in the CaM:SEF2-1mp complex
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