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In principle mainly two properties of the fluorescence emission exhibit the most pronounced signal changes upon conformational changes of the protein
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However, our data indicate that aggregation and in addition at least one of these other mechanisms seems to play a role for the α-amylases with red shifts upon thermal unfolding (i
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Conformational substates in proteins
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Negative wetting force, i
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Figures 6 and 7 reveal that comparatively NC flux gives lower contact angle values than R flux or WS flux regardless of the solder types and temperatures for Cu substrate
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Membrane-bound PLCβ appears stable under pressureIn previous work, we found that pressure increased the reaction rate of PLCδ (Rebecchi et al
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INTRODUCTIONHigh-density lipoproteins (HDLs) are heterogeneous complexes of lipids and specific proteins (termed exchangeable apolipoproteins) that mediate cholesterol removal from peripheral tissues to the liver via the process termed reverse cholesterol transport (Fielding and Fielding, 1995)
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Probing the mechanism of insulin fibril formation with insulin mutants
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Oxidized flavor can also develop during storage even when the ice cream is packaged in opaque containers
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Longer retention times of remains in the stomach also appears to greatly decrease the number of bones that appear in egested pellets (Errington 1930)
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Differences between the values obtained for each culture medium were analysed using the unpaired Student's t-test
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A revolving cast of (young, male) characters show up at the games
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05, or number of ties, F [less than] 1
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. CONCLUSIONS We have used an atomistic EV model, developed in previous work, to show that it is computationally tractable to generate accurate conformational ensembles for proteins in the EV limit
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. Our ability to simulate mimics of denatured-state ensembles will allow us to ask precise questions about the role of the denatured state in the types of applications outlined above
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. (35) suggests that urea, which is chemically equivalent to the main repeating unit in the peptide backbone, can be thought of as a near-perfect solute over the entire solubility range
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. Below the short Newtonian plateau, the viscosity increases with decreasing shear rate
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. Hydrostatic pressure has been used extensively in the past to measure the volume change of protein-protein associations in solution, but it has not yet been used to monitor associations of membrane-bound proteins
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.We used DMA to measure the T^sub g^ and initial modulus for the IPP and EVA polymers (Table IV)
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. In contrast, the evaluation of the spFRET data is modelindependent and the number of distinguishable conformers is directly revealed in the FRET efficiency histograms
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. Dedifferentiation is reversible; the cells may regain their chondrocytic phenotype when cultured in conditions which induce a rounded morphology, such as agarose,11 alginate12-15 or pellet culture
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