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RESULTS AND DISCUSSION The thermal dependence of ACYI activity was determined by measuring the catalytic activity of the samples equilibrated at given temperatures tor 30 min
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This result is, to some extent, consistent with the predicted ACYI structure (17)
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The other reagents used in this study were of the highest purity available
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However, the photo correlation spectroscopy results at pH 6
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Although the other monocations may be involved with DNA binding solely through electrostatic force, there may be some other possibilities with Li+ ion, and the position of Li+ ion in this monocation activity sequence seems to depend strongly on the detection method and experimental conditions
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Hydrostatic pressure has been used extensively in the past to measure the volume change of protein-protein associations in solution, but it has not yet been used to monitor associations of membrane-bound proteins
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Far-U V CD spectra (185-250 nm) were recorded from solutions of 20 µg/ml protein concentration placed in 5-mm-pathlength cells, which are standard condition in our CD experiments
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Implementing MQLRemoving coolant from machining presents challenges related to heat, tool life and chip removal, but certain systems and strategies can address these challenges
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Nevertheless, the stabilizing effect of complexation on backbone and side chains can be unambiguously identified when comparing the buildup curves of the two states (Fig
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) Damore DT, Ramundo ML, Hanna JP, Dayan PS
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The treatment of cartilage damage in the younger patient is a major challenge to the surgeon since articular cartilage has limited potential for intrinsic repair
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) With four patents, this formula uses a water-based acrylic polymer to bind color to the nail and its own specialized clear sealant top coat
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It validated concerns expressed over the years by consumer advocacy organizations, academic physicians, and a few members of Congress
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"We partner with our suppliers to help us bring these events to communities and with specialized companies that staff these screening events and provide materials to educate our pharmacists
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Medco may sit under the radar for many consumers, but it plays a bigger role in the prescription business than most people realize
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The influence of noncognitive variables in student performance
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Over the past three years, those plans have been imposed on millions of patients by pharmacy benefit managers, who extract high retail co-pays from patients if those patients refill their chronic care medications at their neighborhood pharmacy instead of at the PBM-owned mail order pharmacy
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Their wagering and play are better predicted by evidential approaches, which focus on availability of relevant evidence
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. The enzymatic activity was determined by measuring the absorbance at 238 nm accompanied with hydrolysis of the substrate and using the molar absorption coefficient ε^sub 238^ = 185 M^sup -1^cm^sup -1^ as reported by Kordel and Schneider (5,6), except that chloroacetyl-L-leucine was used instead of pure-L-enantiomorph
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. For sequence separations that go beyond this local length scale, chain properties such as R^sub g^
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. Either we could not unambiguously determine the protein-concentration independence of the thermal midpoint from fluorescence measurements (see above, for the values, and Fig
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. Due to the complexity of the investigated proteins (multitryptophan proteins in an ensemble of structurally heterogeneous unfolded states) a quantitative relation between λ^sub max^-values and the extent of aggregation or the compactness of the unfolded state cannot be given
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. Stabilization of recombinant Drosophila acetylcholinesterase
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. Furthermore, the cooling curve of the apoA-2:DMPC sample shows partial protein refolding below 80°C, followed by additional refolding between 32 and 24°C, i
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. Originally conceived as a kinetic theory in which a nucleus of native structure corresponds to the transition-state for folding, the picture now also encompasses the hierarchical folding routes of the diffusion-collision model
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. Bonvin** Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands; and [dagger] Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, Zurich, SwitzerlandSubmitted April 23, 2004, and accepted for publication September 20, 2004
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. The sizes of the primary particles in the gel ranged from 0
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