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. Since ACYI can maintain its dimeric structure at a high GdnHCl concentration of 2 M (18), it seems that the dimerization domain was quite stable against denaturation
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. Energy estimation in protein design
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. However, Tara et al
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. 5 ns pulses, for time-resolved measurements of fluorescence decay and anisotropy
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. In a study that focused on the interaction between PLCβ^sub 2^- and Gβγ-subunits, we found, not surprisingly, that the measured steady-state dissociation constant for the two proteins was equal to the ratio of the on- and off-rates (Runnels and Scarlata, 1998)
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. However, the PLCβ^sub 2^ N-terminal PH domain allows for full activation by Gβγ-subunits (Wang et al
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. It is possible here to provide only a broad overview of the relationships involved
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. It suggests that kinetic strategies for fast folding may be encoded rather generally in nonnative as well as in native interactions
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. Previous studies have focused on morphological features (Herrera and Cousseau, 1996; Rosa and Rosa, 1997; Gosztonyi and Kuba (2)), age and growth (Elias and Burgos, 1988; Fulco, 1996; Gonzalez, 1998), diet (Elias and Rajoy, 1992; Gonzalez, 2002), and reproductive traits, including reproductive season, spawning modality, and age at first maturity (Macchi et al
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. This increase, however, only amounts to Due to the large number of atoms of gp120, the conformational sampling is not yet complete within the 8-ns simulation
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. Since no high-resolution structure of ACYI is available, all of the Trp fluorophores (Classes A and S, I, II, and III) defined previously (34) were included in the fitting procedure
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