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. (33) The second pathway includes ced-1, ced-6, and ced-7 (representing the mammalian homologues LRP, GULP, and ABC-A1 or ABC-A7, respectively)
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. For this test the samples were rapidly frozen in liquid nitrogen and then thawed at ambient temperature
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. Mass normalized fluorescence profiles are shown in Fig
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. ΔR^sub ex^ calculated using Eq
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. Furthermore, the nanosecond-timescale domain motion, present in free CaM, seems to be frozen out, and as far as we know, microsecond-timescale motions are not present in the wraparound binding (10)
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. This loss is compensated via multimerization
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. 2) buffer and taken through 10 alternating freeze-thaw cycles using liquid nitrogen and a 37°C water bath
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. We note that we have found that the affinity between these proteins does not depend on the lipid phase (L
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. An estimation of the T^sub m^ of EIC^sup sc^, based on the fluorescence results (Fig
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