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. The equilibrium unfolding of GR by GdnHCl was monitored by measuring fluorescence emission spectra at various denaturant concentrations
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. Native dimeric GR eluted at a volume corresponding to an apparent molecular weight of ~85 kDa in the calibrated column, indicating a fairly compact dimer structure
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. In the following sections, we show that the differences in Kratky profiles imply that in the EV limit proteins are cigar-shaped, loosely packed coils, with average topologies that are independent of amino acid sequence
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. CONCLUSION In vivo localized MR spectra were recorded from subcellular regions within X
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. The potentials of monocations for DNA compaction in PEG 3000 and 10000 solutions are summarized in Fig
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. We compared the properties of DCVJ with the known amyloid specific dye ThT and found striking similarities, including fluorescence lifetimes and viscosity dependence on fluorescence intensity
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. The phase diagram was constructed by the IR intensity at 1637 cm^sup -1^ vs
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. Evolutionary conservation and variation of protein folding pathways
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. To reduce the variability of fluorescence intensity in frozen samples, due to the poor homogeneity of the medium, the sample was rotated (3 Hz) during the measurement and the signal intensity was integrated for 10 s
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. In the usual space-time windows of IENS experiments, this represents dynamics of larger atomic groups such as protein side chains (3)
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. The optical pathlength was 1 mm and the protein concentration (determined from the absorption at 280 nm) varied from 0
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. Despite these features the overall interaction is highly specific with nanomolar binding strength (16)
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. Li+ ion shows a significantly lower DNA compaction potential than expected based on the trend for other ions, which suggests that monocations with a smaller atomic number are more active in DNA compaction
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