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. These pretransitional conformational changes also resulted in an intermediate with hydrophohic exposure, which could lead to serious aggregation in both chemical denaturants and heat-treatment conditions
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. Statistical Mechanics of Chain Molecules
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. Among a large variety of different methods, such as absorption-, circular dichroism spectroscopy, calorimetry, NMR, and light scattering techniques, in particular fluorescence spectroscopy is a powerful technique to monitor unfolding transitions and to characterize properties of the unfolded state (see for example (3,4))
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. They increase with rf pulse bandwidth and decrease with voxel size
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. Callaghan (New Zealand), P
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. The fluorescence from ANS and Bis-ANS fluorophores show strict environmental dependence and are essentially nonfluorescent in water but are highly fluorescent in hydrophobic environments
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. This affects air incorporation by increasing mix viscosity and causing the defects associated with excess fat agglomeration
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. One may drop the assumption of local forces and allow the protein to "fall" toward the single native state down a "funnel" created by forces whose range permeate the entire volume
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. Atomic insight into the CD4 binding-induced conformational changes in HIV-1 gp120
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. In this case, the apparent crystallization temperature is less than the equilibrium melting temperature, and the monotectic point shifts slightly toward higher polymer concentrations as the cooling rate increases [10]
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. Professor Huizing is also editor-in-chief of the journal Rhinology
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. For procedures, which involve the seeding of cells onto a biomaterial membrane, such as MACI, the provision of a TGF-[beta] containing defined medium during the culture phase prior to implantation is recommended
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