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. Each data point was an average of four accumulations
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. Theory predicts that ensemble-averaged internal distances will scale like ensemble-averaged end-to-end distances such that [left angle bracket]R^sup 2^^sub ij^[right angle bracket]/([left angle bracket]R^sup 2^^sub e^[right angle bracket]|i - j|) = [left angle bracket](r^sub i^ - r^sub j^)^sup 2^[right angle bracket]/([left angle bracket]R^sup 2^^sub e^[right angle bracket]|i - j|)~1 (39)
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. EI proteins are composed of two domains (6,7): a proteolytically inert N-terminal (EIN), and a proteolytically unstable C-terminal domain (EIC)
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. Our pressure studies show that the volume change for this association is twice as high in solution as on membranes
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. For proteins containing N residues, there are of order N^sup 2^ nonnative interactions that may be encountered during a diffusive search, but only of order N interactions that define the native state
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. Of course, it is not impossible to achieve the dimensional reduction we have outlined by using fully native, rather than nonnative interactions
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. Thermal denaturation of rSBA and gSBA Thermal unfolding profiles of both nonglycosylated and glycosylated SBA were monitored by the change in molar ellipticity value at 218 nm at a protein concentration of 13 µM
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. Therefore, the respective unfolded states can have rather different structures (21)
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. For diffusion spFRET measurements, the resulting ribozyme complexes were diluted with annealing buffer (missing NaCl and supplemented with 0
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.Ware JE, Kosinski M, Keller SD
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. (Indeed, China's demand for oil is the leading factor in pushing prices from $10 to $20 a barrel to around $75 a barrel today
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