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. Several NMR studies have been carried out on protein-ligand complexes (reviewed in Palmer (5)), suggesting that both increases and decreases in protein entropy may occur upon ligand-binding
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.But that conclusion irked Darwin
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. Positive crosspeaks could be found at (1649 cm^sup -1^, 1636 cm^sup -1^), and negative crosspeaks were located at (1649 cm^sup -1^, 1617 cm^sup -1^) and (1658 cm^sup -1^, 1617 cm^sup -1^)
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. The supernatant was again subjected to 65% ammonium sulfate fractionation
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. Do harshly denaturing environments such as 8 M urea or 6 M GdnCl mimic perfect solvents? In previous work, we developed a fast and accurate way to generate thermal, self-avoiding distributions for proteins with atomistic detail (48)
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. The proposed torsions are used to compute new Cartesian coordinates for the molecule
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. As for azurin (12), lowering the freezing temperature from -5°C to -13°C increases the number of binding sites but the average affinity is lowered
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.4 ms were applied in a point resolved spectroscopy sequence (PRESS) (26), along with spoiling gradients of 1 ms and 100 mT/m between pulses, which led to a minimum echo time of 6
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. Considering, though, that the Aplysia neuron is an already fully differentiated somatic cell whereas the Xenopus oocyte is a germinal cell containing large fat stores to fuel quick embryonic development, these spectroscopically observed differences in lipid contents turn out to simply reflect, in both cases, physiological adaptations to the function of the respective cell type
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. Model for the effects of time and temperature on the gelation time of BSA gels
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. However, viscosity alone does not give the complete picture, since we also note a significant blue shift in the fluorescence maximum when bound to amyloid fibrils and to prefibrillar misfolded oligomers
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. Results in this study show that the use of ANS fluorescence intensity as a probe of molten globule formation can be complemented with fluorescence anisotropy decay to indicate the quaternary state of the molten-globule protein
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. Moreover, as the phenomenon of protein aggregation appears to reflect certain generic polymeric features of proteins (Fandrich and Dobson, 2002), studying mechanisms of protein aggregation on model systems is extremely useful for a better understanding of the molecular mechanisms of disease-associated amyloidogenesis
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., 2004b), lag phase is a typical feature of protein amyloidogenesis corresponding to the thermodynamically unfavorable nucleation process (Bouchard et al
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. Status of the coastal black rockfish stocks in Washington and northern Oregon in 1994
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.Yamada, J, and M
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. In order to suppress non-specific binding of IgG, the sections were blocked with normal rabbit serum (Dako AJS, Denmark), diluted 1:5 in TBS for 20 minutes
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.--Pharmacology/ Pharmaceutical Sciences Toxicology Neurosciences* NEBRASKACreighton University School of Pharmacy and Health Professions M
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. The South Oaks gambling screen (SOGS): A new instrument for the identification of pathological gamblers
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