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It is known that apomyoglobin is stable in its native state at neutral pH and 30 deg C
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Moreover, the strategies used in this research might also facilitate the characterization of the potential pretransitional changes in the thermal transitions of other thermostable or thermophilic proteins
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The supernatant was again subjected to 65% ammonium sulfate fractionation
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CONCLUSIONS In summary, our work shows that the nonglycosylated form of SBA unfolds via a non-two-state pathway, in contrast to the glycosykued naturally occurring protein
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The relationship observed between reduced protein flexibility and increased thermal stability is in line with other experiments on trehalose-coated myoglobin (56) and on lysozyme in glycerol (57,58)
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Although the differences in the χ^sup 2^ residuals between M2II models with different τ^sub is^ are not statistically significant, it is safe to conclude that there is a slow timescale motion present with a correlation time of roughly 2
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Since it has been widely recognized that changes in the conformation of DNA into higher density forms is the result of DNA negative charge neutralization (Bloomfield, 1991), the activity of cationic molecules in neutralizing DNA negative charge (through binding with DNA phosphates) has been associated with the potential to induce DNA phase separation processes, such as compaction, condensation, aggregation, and precipitation, also without clear distinctions between these different processes
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, upon various therapeutic treatments
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For DNS-PE-labeled membranes, the labeled lipid was added to the chloroform lipid solution before drying
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When considering all protein atoms, the number of atoms in gp120 becomes too large to obtain sufficient sampling in the timescale of our simulation (10 ns); the buildup curves have not yet converged (Fig
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The inability of two- and three-way junction ribozymes to fully populate a docked conformation reveals the importance of correct helical junction geometry as well as loop elements for effective ribozyme folding
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However, further analyses showed that the outcome of the previous trial had a marginally significant effect on the change in bet size, F(1, 11) = 4
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