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. 5 M GdnHCl), reflecting exposure of tryptophan residues to the aqueous medium (Fig
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. It is worth mentioning here that our efforts to cleave the intact glycoprotein using α-mannosidase failed repeatedly
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. and those of Wilkins et al
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. Our results show that dimeric EI^sup sc^ acquired a folded conformation at physiological pH: however, 8-anilino-1-naphthalene-sulfonic acid (ANS)-binding experiments, FTIR, and CD measurements suggested that the protein populated a molten-globule-like species at low pH
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. Fluorescence accessory was used to record 90° light scattering simultaneously with the CD
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. When attempting to elucidate a plausible mechanism of formation of these early, yet relatively thick fibrils, it must be taken into account that the fibrils' ends are not frayed, i
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. Time-resolved FRET (trFRET) measurements were subsequently used to resolve and quantify the equilibrium distribution of extended and docked ribozyme conformers (N
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. A 100 µl sample of Hoechst (1 µg/ml) was added to each of the wells
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.5 M GdnCl, as described previously (18)
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. We speculate that under folding conditions, it is the typological distinction between the native and highly denatured states that provides part of the driving force for folding via collapse and symmetry breaking
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. All α-amylase structures bear multiple tryptophan residues more or less equally distributed over the whole structure (see Table 1 and Fig
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. At (180 µm)^sup 3^, our voxel size, a gradient field strength of 1
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