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5 M GdnHCl), reflecting exposure of tryptophan residues to the aqueous medium (Fig health woman .
It is worth mentioning here that our efforts to cleave the intact glycoprotein using α-mannosidase failed repeatedly health woman .
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Our results show that dimeric EI^sup sc^ acquired a folded conformation at physiological pH: however, 8-anilino-1-naphthalene-sulfonic acid (ANS)-binding experiments, FTIR, and CD measurements suggested that the protein populated a molten-globule-like species at low pH health woman .
Fluorescence accessory was used to record 90° light scattering simultaneously with the CD health woman .
When attempting to elucidate a plausible mechanism of formation of these early, yet relatively thick fibrils, it must be taken into account that the fibrils' ends are not frayed, i health woman .
Time-resolved FRET (trFRET) measurements were subsequently used to resolve and quantify the equilibrium distribution of extended and docked ribozyme conformers (N health woman .
A 100 µl sample of Hoechst (1 µg/ml) was added to each of the wells health woman .
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