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NH^sub 2^-terminal sequence truncation decreases the stability of bovine rhodanese, minimally perturbs its crystal structure, and enhances interaction with GroEL under native conditions
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This fold comprises a six-stranded hack β-sheet, a curved seven-stranded trout β-sheet and a rive-stranded sheet that forms the roof of the molecule (14,15)
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In our EV model, there is only one free parameter, namely the exponent n
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Second, unlike hard-sphere potentials, which stipulate that all sterically allowed conformations are isoenergetic, soft-core potentials encode the requisite conformational specificity (48,53,58)
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Hvdrodynamic radii of native and denatured proteins measured by pulse field gradient NMR techniques
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At lower pHs an increase in ionic strength screens the electrostatic repulsion of positive charges on the monomer and stabilizes the dimer (25,26)
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Bound to aggregated TTR, the fluorescence lifetime of both components have increased and the relative amplitude of the slower component has increased (Table 3), well in accordance with the massive increase in quantum yield compared to when bound to native TTR (Fig
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Previous studies have pointed out that-at low pH and in the absence of co-solvents-the dimeric insulin must undergo temperature-induced monomerization before the actual aggregation (Nettleton et al
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One can, for example, resolve the entropic contributions of hydrophilic and hydrophobic residues to protein-protein binding, which is experimentally impossible
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4 D), on the other hand, a smooth continuation at 8 ns of the configurational entropy buildup is observed when the complex trajectory is appended to the free one (solid line), whereas a slight stepwise increase is visible when the trajectories are appended in the reverse order (dashed line)
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Results and DiscussionOne isotactic polypropylene (IPP) and two polyethylene-co-vinyl acetate (EVA) toners were produced for textile xerographic printing by means of TIPS
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The smallest particle size (22 µm) occurred with a 10 wt% solution of PP3825 at 130°C dissolving temperature, so we used these conditions for the subsequent tests
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However, their methods may have limitations if samples are preserved in formaldehyde for several years or if DNA must be extracted from numerous samples
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Break it down by discussing the meaning of the individual words--"social" and "neuroscience
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. Furthermore the intensity is decreasing during the heating process with an additional decrease in the transition region
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. Finally, proteins-which represent one-third of the oocyte's dry mass, far exceeding the contribution of ribonucleic acids (41)-have been shown to bind large quantities of diminazene in other systems (see Mamman et al
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.SUPPLEMENTARY MATERIALAn online supplement to this article can be found by visiting BJ Online at http://www
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. The body was elongate and relative body depth remained fairly constant throughout development
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. 1 and then normalized by dividing it by the number of atoms of each residue
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.6% of all larvae extruded, respectively (Table 5)
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. These distances are in agreement with the corresponding values obtained from the present spFRET experiments (see Results section above)
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. One group was cultured further in DMEM/ITS+/TGF-[beta]1 and the other was cultured in DMEM supplemented with 10% (v/v) foetal calf serum (FCS, Life Technologies, Paisley, UK)
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. The Student-Life Stress Inventory was used in this study since it's potential respondent burden was acceptable, it was easy to self-administer and it had an acceptable previously reported internal consistency estimate (Cronbach's alpha = 0
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