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According to one notation on the Carter Center website, for example, King Fahd of Saudi Arabia donated $7
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. Although gSBA was seen to demonstrate very high conformational stability, rSBA exhibits comparatively much lower firmness to resist the structural perturbation induced by the denaturant guanidinium hydrochloride
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. thanks the Council of Scientific and Industrial Research, India, for the award of Senior Research Fellowship
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. It has been suggested that thermal fluctuations are correlated with and act as a lubricant for slower, functional motions taking place on the microsecond or longer timescales (6)
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. All measurements were performed at room temperature
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. By comparing the volume change of PLCβ-Gβγ alone and in the presence of Gα^sub s^(GDP), we can estimate the volume of activation of PLCβ^sub 2^ by Gβγ-subunits
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. Time-resolved fluorescence resonance energy transfer: a versatile tool for the analysis of nucleic acids
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. 1b) for pellets cultured for two days in DMEM/ITS+/TGF-[beta]1 and for up to 12 days in either DMEM/ITS+/TGF-[beta]1 or DMEM/10%FCS
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. Since no high-resolution structure of ACYI is available, all of the Trp fluorophores (Classes A and S, I, II, and III) defined previously (34) were included in the fitting procedure
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. These data suggest that the percentages of helical structure in EI^sup sc^ at the extremes of pH were increased
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. The protein purity assessed by SDS gel electrophoresis was 95%
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. Although the exact time of disk reconstitution varies for apA-2 from different pools, our data consistently show that the vesicle-to-disk conversion after heat denaturation of apoA-2:DMPC disks is a slow reaction that takes hours to complete at 24°C
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