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. Based on a two-component mixture theory, we obtained the following hydration numbers per protein molecule: 590 +/- 65 for N, 630 +/- 73 for A, and 1110 +/- 67 for U^sub A^
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. Dissecting the Pretransitional Conformational Changes in Aminoacylase I Thermal DenaturationSu, Jing-TanABSTRACT Aminoacylase I (ACYI) catalyzes the stereospecific hydrolysis of L-acylamino acids and is generally assumed to be involved in the final step of the degradation of intracellular N-acetylated proteins
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. As noted by Chan and Dill in their influential review (37), theories drawn from the polymer physics literature (38-44) are well-suited to describe heterogeneous conformational ensembles such as those of denatured slates
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. In principle, unfolded states are rather heterogeneous (26-28) and in addition the mechanisms leading to unfolded states are rather different for thermal and GndHCl-induced unfolding (1)
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. Figure 11 represents the morphology of Sn-Ag-Cu-Bi solder after dipping the Cu and Ni substrate into it
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